GSM4932637: 9_16_c_LE8_4421; Dictyostelium discoideum; RNA-Seq - SRA

SRX9567335: GSM4932637: 9_16_c_LE8_4421; Dictyostelium discoideum; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 19.9M spots, 3G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)

Study: RNAseq and quantitative proteomic analysis of Dictyostelium knock-out cells lacking the core autophagy proteins ATG9 and/or ATG16

PRJNA680492 • SRP293794 • All experiments • All runs

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Purpose: Global survey of the transcriptome and comparison to the proteome in autophagy-deficient strains of D. discoideum. Results: Our RNAseq analysis of ATG9?, ATG16? and ATG9?/16? cells revealed major transcriptional changes in a large number of genes in comparison to AX2. Conclusions: Our study provides the first combined transcriptome and proteome analysis of ATG9?, ATG16? and ATG9?/16? cells and adds to our knowledge of functions of these proteins in Dictyostelium discoideum. Overall design: Total RNA from vegetative Dictyostelium discoideum cells (AX2 wild-type and ATG9?, ATG16?, ATG9?/16? mutant strains) was isolated; sequencing and analysis was as described in Karow et al. Cells, 9(5), E1179. n=9, Illumina Genome Analyzer IIx

Sample: 9_16_c_LE8_4421

SAMN16881646 • SRS7758336 • All experiments • All runs

Organism: Dictyostelium discoideum

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: total RNA extraction was carried out using the Qiagen RNeasy® Mini Kit according to the manufacturer's instructions Illumina RNASeq library Protocol Libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit

Experiment attributes:

GEO Accession: GSM4932637

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 19.9M spots, 3G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR13125310	19,854,515	3G	1.3Gb	2021-05-19

ID:: 12498750

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